Fig. 3.

Let-7b promotes cell differentiation in HaCaT. a Overexpression of let-7b in HaCaT transfected with let-7b mimic compared to HaCaT transfected with let-7b negative control (miR-NC). b Western blot and Real-time PCR analysis of early and late epidermal differentiation markers cytokeratin 1 (CK1), cytokeratin 10 (CK10), filaggrin, Involucrin and Loricrin. c HaCaT cells were transfected with let-7b inhibitor and inhibitor negative control (“InNC”). d Western blot and Real-time PCR analysis of early and late epidermal differentiation markers. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01