Fig. 5.

Let-7b suppresses expression of IL6 in a keratinocyte-specific let-7b transgenic mouse model. a qRT-PCR analysis of IL-6 expression in lesioned skin samples from IMQ-induced wild-type mouse model. b qRT-PCR revealed down-regulation of IL6 expression in primary keratinocytes from wild-type and transgenic mice. c qRT-PCR analysis revealed IL6 level of wild-type and transgenic mice treated with IMQ at days 3 and 5. d Immunohistochemical localization of IL6 protein 5 days after mice were treated with IMQ (n = 5). Histograms represent the average ± SD from three independent assays. *P < 0.05; **P < 0.01;***P < 0.01. Scale bars, 100 μm