Figure 6.

Concordance of SIRT2 mRNA and protein expressions in NSCLC. (A). SIRT2 immunohistochemical staining results in normal lung tissues (n=3) and lung cancer tissues (n=12). The images were obtained from the Human Protein Atlas.⁴³ (B). SIRT2 mRNA expression in normal lung tissues (n=110) and lung cancer tissues (n=1017) in the TCGA NSCLC cohort. (C–E). SIRT2 mRNA expression detected by qRT-PCR (C) and protein expression detected by Western blotting (D) in seven lung cancers and paired adjacent normal tissues (n=7); consistency of SIRT2 mRNA and protein expression were explored by Pearson’s correlation analysis (E). These were re-analyses results of Li et al’s report.⁴⁴ (F). Associations of SIRT2 mRNA with protein expressions in lung cancers. SIRT2 protein expression and mRNA expression data were extracted from proteomics and gene microarray profiles, respectively, which was reported by Stewart et al.⁴⁶

Abbreviations: SIRT2, sirtuin 2; N, normal; T, tumor; qRT-PCR, quantitative real-time polymerase chain reaction.