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. 2018 Sep 6;19:339–353. doi: 10.1016/j.redox.2018.09.006

Fig. 2.

Fig. 2

AGEs treatment induced mitochondrial dysfunction and cytochrome c release via prolonged mPTP activation and Bax translocation at mitochondria. The human NP cells were treated as described in Fig. 1. (A) Representative fluorescent images with calcein staining and quantitative analysis of fluorescence intensity using Image-Pro Plus 6.0 for mPTP. Green fluorescence indicated calcein signals. Cell nuclei are stained by DAPI. *p < 0.05 versus PBS (36 h). (B) The quantitative analysis of the shift of red fluorescence (x axis) to green fluorescence (y axis) for MMP and representative scatter plots of flow cytometry by JC-1 staining. *p < 0.05 versus PBS (36 h). #p < 0.05 versus 0 h. (C) Representative western blotting assay and quantitation of the ratio of Bcl-2/Bax protein in mitochondrial extracts. *p < 0.05 versus PBS (36 h). #p < 0.05 versus 0 h. (D) Representative western blotting assay and quantitation of the level of cytochrome c (Cyt-c) in mitochondrial and cytoplasmic extracts. *p < 0.05 versus PBS. Scale bar: A, 60 µm.