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Cross-link yield as a function of the amount of mutant BRAF sequence present in mixtures of mutant and wild-type duplexes. Samples run in triplicate containing various proportions of mutant and wild-type BRAF duplexes (21 bp) were denatured by warming at 50–70 ˚C in the presence of the second-generation probe, cooled, incubuated at 37 ˚C, and the yield of interstrand cross-link assessed by gel electrophoretic analysis.
