Table 1.

RNASseq RPKM values from laser captured microdissected human pancreatic adenocarcinoma cancers and patient-matched normal pancreatic acinar tissue or normal pancreatic ducts.

Gene	Function	Tumor n = 16	Normal duct n = 16	Tumor / Normal duct	Tumor n = 5	Normal acinar n = 5	Tumor / Normal acinar	p-value (paired t-test)
Genes for pyruvate uptake and metabolism to lactate
SLC2A1  (GLUT1)	PM glucose transporter	97.09	12.75	7.61	76.10	0.41	184.05	< 0.0001
HK2	Hexokinase II	32.67	8.11	4.03	68.15	0.77	89.01	< 0.0001
SLC16A1  (MCT1)	PM pyruvate/lactate transporter	12.38	4.46	2.78	24.83	1.29	19.26	< 0.0001
SLC16A3  (MCT4)	PM pyruvate/lactate transporter	21.27	7.85	2.71	5.53	0.05	113.47	< 0.0001
LDHA	Lactate dehydrogenase A	351.6	142.31	2.47	305.27	20.67	14.77	< 0.0001
FOXM1	LDHA-activating transcription factor	6.17	1.45	4.27	2.21	0.12	18.40	0.0004
Other competing pyruvate metabolic pathway genes
BRP44L	Inner mito. Membrane pyruvate carrier	26.05	29.48	0.88	14.37	10.63	1.35	0.34
GPT2	Glutamate-pyruvate transaminase	7.79	12.57	0.62	8.21	32.91	0.25	0.0015
PDHA1	Mitochondrial pyruvate dehydrogenase	17.86	16.61	1.08	19.29	16.58	1.16	0.1585
PDHP	Mitochondrial pyruvate dehydrogenase	35.88	36.23	0.99	28.75	16.60	1.73	0.4035

Aerobic glycolysis genes are highly upregulated in pancreatic cancer. Other competing pyruvate metabolic pathway genes are downregulated or largely unchanged. RPKM: average reads per kilobase per million reads. PM: plasma membrane.