Skip to main content
. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2018 Aug 13;102(19):8437–8446. doi: 10.1007/s00253-018-9283-z

Figure 1.

Figure 1.

Schematic diagram depicting (A) the replacement of salA-C by Red/ET-mediated recombination with the attB-containing cassette; (B) subsequent removal of the resistance marker by FLP-mediated excision; and (C) replacement of the fosmid backbone chloramphenicol resistant gene cat with acc(3)IV (ApraR) and oriT of RK2 to generate fosmid pMXT11 to simultaneously knock-in the attB attachment site and knock-out salA-C in S. tropica CNB-440.