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Detection of thiolactomycin and its analogues in various producing strains by HPLC. (A) Schematic illustrating the thiolactomycin (tlm) biosynthetic gene cluster. HPLC chromatograms of ethyl acetate extracts from (B) S. tropica CNB-4401/tlm, (C) S. tropica CNB-4401, (D) S. pacifica CNS-863, and (E) S. coelicolor M1152/tlm. Detection at 239 nm. (F) Structures of thiolactomycin (1), 10-methyl thiolactomycin (2), 11-methyl thiolactomycin (3), and thiotetromycin (4).
