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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: Cancer Res. 2018 Aug 2;78(18):5431–5445. doi: 10.1158/0008-5472.CAN-17-3811

Fig. 5. RAB38 regulates melanoma metastasis by mediating the expression levels and activities of MMP2 and MMP9. (A).

Fig. 5

RT-qPCR assays showed decreased expression levels of MMP2 and MMP9 in WM-266-4 cells with siRAB38 knockdown as compared to non-targeting siRNA control. Error bars represent mean ± standard error of the mean (SEM) (n = 3); (B) Gelatin zymography assays revealed diminished enzymatic activities of MMP2 and MMP9 in WM-266-4 cells with siRAB38 knockdown as compared to non-targeting siRNA control; (C) Quantification results for gelatin zymography assays shown in (B). Error bars represent mean ± standard deviation (n = 3). (D) Real-time quantitative PCR (RT-qPCR) assays showed increased expression levels of MMP2 and MMP9 in FLAG-RAB38-expressing WM-115 cells as compared to empty vector control. Error bars represent mean ± SEM (n = 3); (E) Gelatin zymography assays revealed elevated enzymatic activities of MMP2 and MMP9 in FLAG-RAB38-expressing WM-115 cells as compared to empty vector control; (F) Quantification results for gelatin zymography assays shown in (E). Error bars represent mean ± standard deviation (n = 3). The p values for all figures are as follows: “*”, 0.01 < p < 0.05; “**”, 0.001 < p < 0.01; “***”, p < 0.001. The p values were calculated by using a paired, two-tailed Student’s t test.