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. 2018 Sep 6;24:6218–6228. doi: 10.12659/MSM.908510

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© Med Sci Monit, 2018

This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

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Allograft inflammatory factor-1 (AIF-1) protein and mRNA expression in hepatocellular carcinoma (HCC) and non-cancerous liver tissues. (A) Representative immunohistochemical analysis of allograft inflammatory factor-1 (AIF-1) expression levels in HCC and corresponding adjacent non-cancerous liver tissues (adjacent). (B) The expression of AIF-1 protein was detected in 104 HCC tissue specimens (C) and corresponding adjacent non-cancerous liver tissues (N) by Western blotting analysis. Glyceraldehyde phosphate dehydrogenase (GAPDH) was used as an internal control. Representative Western blots show an enhanced expression of AIF-1 protein in HCC tissue specimens. (C, D) Western blotting and qPCR analysis were conducted to measure the protein and mRNA levels of AIF-1, respectively, in 104 HCC and corresponding adjacent non-cancerous liver tissue samples. * p<0.05 versus HCC tissues.