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. 2018 Sep 15;13:23. doi: 10.1186/s13064-018-0120-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Analysis of retinal slice survival and proliferation. Retinal slice cultures stained with Zombie Red™ dye after 0 h (a and c) and 16 h (b and d) in culture. Higher magnifications of boxed region in A-D (E-H). The quantification of Zombie Red+ cells (ZR+ pixels/retinal area) showed no significant differences between 0 h and 16 h in culture (i) or between tissue exposed to laser versus unexposed (j). n = 9 per group. EdU labeling (6-h pulse) and quantification of RPCs in P0 and P1 retinae compared to P0 retinal slices time lapse cultures (k-n). n = 3 per group. Error bars represent SE. Abbreviations: NBL (neuroblastic layer), IPL (inner plexiform layer), GCL (ganglion cell layer)