Figure 3:

Computing transfer free energy of Gly₄ from water to urea solutions. A z-dependent concentration profile of urea (broken red lines, right axis) has been built up by applying a constant external potential to the urea molecules. The symbols indicate the corresponding free energies F(z) for individual Gly₄ molecules determined from the population density, as indicated in the legend. The black line is interpolated from the experimental data.²⁶