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. 2018 Sep 10;9:2038. doi: 10.3389/fimmu.2018.02038

Figure 2.

Figure 2

Selective deficiency of CD8αα+γδ T cells in FADD-DN mice. (A) Representative FACS analysis of CD8αα+ (CD8α+CD8β−) and CD8αβ+ (CD8α+CD8β+) populations in the IELs from WT and FADD-DN mice. Statistical analysis of CD8αα+ and CD8αβ+IELs from indicated groups (n = 6 per group) is on the right. Each dot represents one mouse of each experimental group. **P < 0.01. (B) CD8αα+ cells gated from (A) were analyzed for the expression of TCRαβ and TCRγδ, distinguished into two subsets: CD8αα+ αβT (CD8αα+TCRαβ+TCRγδ−) and CD8αα+ γδT (CD8αα+TCRαβ−TCRγδ+). (C) CD8αβ+ cells gated from (A) were analyzed for the expression of TCRαβ and TCRγδ, distinguished into two subsets: CD8αβ+αβT (CD8αβ+TCRαβ+TCRγδ−) and CD8αβ+ γδ T (CD8αβ+TCRαβ−TCRγδ+). (D) Statistic analysis of the percentages of indicated subsets among total IELs from WT and FADD-DN mice (n = 6 per group). Each dot represents one mouse of each experimental group. *P < 0.05. (E) Time-dependent changes of the percentage of CD8αα+TCRγδ+ subset in the IELs from WT and FADD-DN mice analyzed by FACS. Error bars reflect S.E.M. (n = 3 per group). (F) Time-dependent changes of the percentage of CD8αα+TCRβ+ subset in the IELs from WT and FADD-DN mice analyzed by FACS. Error bars reflect S.E.M. (n = 3 per group). Data was calculated from three mice per group.