Table 1.

Primer pairs of DEGs used for qRT-PCR validation.

Gene	Primer (5′→3′)	Product size (bp)	Primer position
FMO2	F: CAGGTATCCAGAAGTTCAAA	105	ENSOART00000013523.1: 488–507
	R: CTGAGTTTCCTATTCCAATCA		ENSOART00000013523.1: 572–592
PENK	F: TGGGAGATGAAACCAAAGAG	171	ENSOART00000021977.1: 446–465
	R: CAGGAACTTCCTTGGAGTAA		ENSOART00000021977.1: 597–616
DPT	F: GAGTGGCAATTTTACTGCTG	132	ENSOART00000010048.1: 379–398
	R: CCCTCGCATATAATAATCATAATTG		ENSOART00000010048.1: 486–510
RASD1	F: CTACCAACTGGACATCCTC	190	ENSOART00000001878.1: 421–439
	R: CTCCTTGGTCTTGTTCTTTAG		ENSOART00000001878.1: 590–610
MID1IP1	F: CGACACCTACAACCAGAAG	78	ENSOART00000020945.1: 15–33
	R: GTCTGGTCCATGTTGTTCA		ENSOART00000020945.1: 74–92
PRKAR2B	F: CTCCAGTAATAAACCGATTTAC	110	ENSOART00000005398.1: 283–340
	R: GTCAGTTTTGGGATGTATAATC		ENSOART00000005398.1: 371–392
ELOVL3	F: TCGGTATCCTGGCTTATATC	117	ENSOART00000019158.1: 647–666
	R: GGAAGAACTTGACAAAGAGA		ENSOART00000019158.1: 744–763
PDK4	F: GGAACTGATGCTATCATCTA	81	ENSOART00000003809.1: 1066–1085
	R: GAAGGCTGATTTGTTAAAGA		ENSOART00000003809.1: 1127–1146
PLIN2	F: CTCAGGATAAGCTCTATCTG	73	ENSOART00000015469.1: 830–849
	R: TGGGATTCATCTGTATCATC		ENSOART00000015469.1: 883–902
TCAP	F: CTGCAGGAATACCAGCTG	189	ENSOART00000012585.1: 232–249
	R: CAGCTGCTTGGTGATCTC		ENSOART00000012585.1: 403–420
SLC22A4	F: ACCCAGACGTTATATCATAG	97	ENSOART00000016553.1: 1266–1285
	R: GATGGACAAGAAGTTGTAAC		ENSOART00000016553.1: 1343–1362
LTF	F: GCCATATAATTTCCATAATTTCATC	165	ENSOART00000009392.1: 4121–4145
	R: TTGGGTGTTTCAGAAAGTAA		ENSOART00000009392.1: 4266–4285
ADGRG3	F: GCTTGTTTCTCCTGAATCTG	176	ENSOART00000000666.1: 953–972
	R: GGTGTTAAAGACCTTGATGA		ENSOART00000000666.1: 1109–1128
LEPR	F: AAGGGTTCTATTTGTATTAGTGA	118	ENSOART00000011314.1: 2909–2931
	R: GGGTGGCATATTTAACAGAG		ENSOART00000011314.1: 3007–3026
GAPDH	F: CACTCACTCTTCTACCTT	91	NM_001190390.1: 900–917
	R: GCCAAATTCATTGTCGTA		NM_001190390.1: 973–990