Table 1.
Free-running activity rhythms in DD.
| Genotype | n | Period (h) ± SEM | Power ± SEM | R (%) |
|---|---|---|---|---|
| w1118 | 62 | 24.2 ± 0.1 | 151.6 ± 6.6 | 90.3 |
| CCHa1SK8 | 32 | 24.2 ± 0.1 | 178.3 ± 8.5∗ | 100 |
| CCHa1MI09190 | 31 | 23.7 ± 0.1∗∗ | 123.9 ± 7.8∗ | 90.3 |
| tim/+ | 32 | 24.6 ± 0.1 | 214.5 ± 8.6 | 100 |
| clk856/+ | 31 | 24.1 ± 0.1 | 143.7 ± 8.6 | 83.9 |
| +/CCHa1RNAi | 32 | 24.5 ± 0.1 | 211.4 ± 8.7 | 96.9 |
| +/CCHa1-RRNAi | 32 | 24.5 ± 0.1 | 153.5 ± 6.7 | 96.9 |
| tim > CCHa1RNAi | 32 | 24.2 ± 0.1 | 175.8 ± 11.7∗ | 96.9 |
| clk856 > CCHa1RNAi | 63 | 23.8 ± 0.1∗∗ | 151.4 ± 5.7 | 95.2 |
| tim > CCHa1-RRNAi | 32 | 23.5 ± 0.1∗∗ | 173.4 ± 9.2 | 96.9 |
DD, constant darkness; CCHa1, CCHamide1; SEM, standard error of the mean. The free-running period, power, and rhythmicity (R) was determined from data of days 1–10 of DD using χ2-periodogram analysis. CCHa1 mutants and RNAi strains were compared with the corresponding control lines, e.g., UAS or Gal4 control lines. If period or power was significantly different from both of the corresponding control lines, it was designated as statistically significant. One-way ANOVA with Tukey’s multiple comparison test was used after testing for the normal distribution of data using the Kolmogorov–Smirnov test; ∗p < 0.05, ∗∗p < 0.01.