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. 2018 Sep 10;9:1248. doi: 10.3389/fpls.2018.01248

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Copyright © 2018 Roustan, Roustan, Weidinger, Reipert, Kapusi, Shabrangy, Stoger, Weckwerth and Ibl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence of HvPDIL1-1 with anti-AtPDIL1-1 at 6, 12, and ≥20 DAP. Aleurone (AL), subaleurone (SA), starchy endosperm (SE). Arrows indicate PBs with a smaller diameter, open arrows indicate PBs with a larger diameter and arrowheads the specific signal from the protein matrix at the periphery of the starch granules (asterisks). See the labeling of the plasma membrane in starchy endosperm (SE) at 12 DAP and in aleurone (AL) and subaleurone (SA) at ≥20 DAP (open arrowheads). Note the weak signal in aleurone (AL) at ≥20 DAP. Scale bar = 50 μm.