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. 2018 Sep 10;8:316. doi: 10.3389/fcimb.2018.00316

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Copyright © 2018 Hijazi, Visaggio, Pirolo, Frangipani, Bernstein and Visca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of heme-uptake systems on A. baumannii susceptibility to GaPPIX. (A) Schematic representation of the two gene clusters for heme uptake, hemT and hemO, of A. baumannii ACICU. Coding sequences are shown as arrows (not to scale) oriented according to the predicted direction of transcription. (B) Top; presence (+) or absence (-) of heme clusters in different A. baumannii strains (++, overexpression from the multicopy plasmid pVRL1hemO). Middle; GaPPIX disk diffusion assay in RPMI-HS agar plates inoculated with different A. baumannii strains. Bottom; MICs of GaPPIX for different A. baumannii strains in RPMI-HS.