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. 2018 Sep 10;9:1023. doi: 10.3389/fphar.2018.01023

FIGURE 1.

FIGURE 1

Schematic illustration of the experimental schedule. (A) Protocol overview of the study. After 2 days of behavioral training (rotarod and pole tasks), all rats were administered drug injection on day 0. According to the time of the behavioral test, (starting on day 1, 5, or day 10 after drug administration) they represented here in three parts. (B) After drug application, six separated animal groups (Saline, Methadone, Naloxone, M/N-Sedate, Saline control-Day 1, and M/N-Day 1) were used to evaluate different behavioral tests including Y-Maze, Rotarod test and Pole test on day 1 after drug administration and Novel object recognition (NOR) test from day 1 to day 4 post-treatment. (C) In two other separated groups (Saline control-Day 5, M/N-Day 5), after administration of saline alone or methadone + naloxone on day 0, Y-Maze, Pole test, and Rotarod test were carried out on day 5 followed by NOR test from day 5 to day 8. (D) In Saline control-Day 10 and M/N-Day 10 groups, 10 days after administration of saline alone or methadone + naloxone, Y-Maze, Pole test and Rotarod test were carried out on day 10 followed by NOR test from day 10 to day 13.