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. 2018 Sep 10;9:1023. doi: 10.3389/fphar.2018.01023

FIGURE 2.

FIGURE 2

(A) Spontaneous alternation behavior and (B) Total number of arm entries were recorded in different groups, including; M/N-treated groups (a single dose of naloxone was administered after methadone overdose in apnea stage, in animals which experienced apnea and spontaneous alternation behavior was evaluated either on day 1, 5, or 10 day post-resuscitation; M/N-Day 1 (n = 6), M/N-Day 5 (n = 6) and M/N-Day 10 (n = 6) groups and their respective Saline control groups; n = 6 right panel), M/N-Sedate (n = 8; a single dose of naloxone was administered following methadone overdose, immediately in the initial stage of sedation, so behavioral evaluation was carried out only 1 day after drug administration), Saline; n = 12, methadone; n = 8 and naloxone; n = 7 (saline, methadone or naloxone were administered alone in separated groups in which spontaneous alternation behavior and the number of arm entries were recorded only 1 day after drug administration during an 8-min trial in adolescent rats) groups. Animals received methadone (15 mg/kg; i.p.) or naloxone (2 mg/kg; i.p.) alone or both (apnea groups) in a single dose. Each bar shows the mean ± SEM for 6–12. P < 0.05 different from the Saline group. +P < 0.05 and ++P < 0.01 different from their respective Saline control groups.