Table 2.
Primers used for quantitative real-time PCR
| Gene | Primer 1 | Primer 2 |
|---|---|---|
| Acacb | CCTTTGGCAACAAGCAAGGTA | AGTCGTACACATAGGTGGTCC |
| Acly | AAGAAGGAGGGGAAGCTGAT | TCGCATGTCTGGGTTGTTTA |
| Actb | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| Arg1 | CTCCAAGCCAAAGTCCTTAGA | AGGAGCTGTCATTAGGGACATC |
| Cd68 | TGTCTGATCTTGCTAGGACCG | GAGAGTAACGGCCTTTTTGTGA |
| Csf1r | TGTCATCGAGCCTAGTGGC | CGGGAGATTCAGGGTCCAAG |
| Dgat2 | GCGCTACTTCCGAGACTACTT | GGGCCTTATGCCAGGAAACT |
| Emr1 | TGACTCACCTTGTGGTCCTAA | CTTCCCAGAATCCAGTCTTTCC |
| Fasn | GGAGGTGGTGATAGCCGGTAT | TGGGTAATCCATAGAGCCCAG |
| Fgf21 | CAAGACACTGAAGCCCACCT | CACCCAGGATTTGAATGACC |
| G6pase | CGACTCGCTATCTCCAAGTGA | GTTGAACCAGTCTCCGACCA |
| Gapdh | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| Glut1 | CAGTTCGGCTATAACACTGGTG | GCCCCCGACAGAGAAGATG |
| Glut4 | GTGACTGGAACACTGGTCCTA | CCAGCCACGTTGCATTGTAG |
| Gpat1 | ACAGTTGGCACAATAGACGTTT | CCTTCCATTTCAGTGTTGCAGA |
| Gsta4 | TGATTGCCGTGGCTCCATTTA | CAACGAGAAAAGCCTCTCCGT |
| Gusb | GTGGTATGAACGGGAAGCAAT | AACTGCATAATAATGGGCACTGT |
| Il6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
| Jun | CCTTCTACGACGATGCCCTC | GGTTCAAGGTCATGCTCTGTTT |
| Lipe | GATTTACGCACGATGACACAGT | ACCTGCAAAGACATTAGACAGC |
| Nqo1 | GGCATCCTGCGTTTCTGTG | GGTTTCCAGACGTTTCTTCCAT |
| Nrf2 | CCATTTACGGAGACCCACCGCCTG | CTCGTGTGAGATGAGCCTCTAAGCGG |
| P2rx5 | CTGCAGCTCACCATCCTGT | CACTCTGCAGGGAAGTGTCA |
| Pat2 | GTGCCAAGAAGCTGCAGAG | TGTTGCCTTTGACCAGATGA |
| Pepck | CTGCATAACGGTCTGGACTTC | CAGCAACTGCCCGTACTCC |
| Pgc1a | CCCTGCCATTGTTAAGACC | TGCTGCTGTTCCTGTTTTC |
| Plin1 | GGGACCTGTGAGTGCTTCC | GTATTGAAGAGCCGGGATCTTTT |
| Pnpla2 | CAACGCCACTCACATCTACGG | GGACACCTCAATAATGTTGGCAC |
| Prdm16 | CAGCACGGTGAAGCCATTC | GCGTGCATCCGCTTGTG |
| Tbp | AAGAGAGCCACGGACAACTG | TACTGAACTGCTGGTGGGTC |
| Tnfa | CATCTTCTCAAAATTATTCGAGTGACAA | TGGGAGTAGACAAGGTACAACCC |
| Ucp1 | CACTCAGGATTGGCCTCTACG | GGGGTTTGATCCCATGCAGA |