Fig. 13.

Expression of wild-type NKCC1 is reduced by ~50% in brain of NKCC1-DFX heterozygote mice. A: Western blot analysis of oocyte lysates from Fig. 2A with a NH₂-terminal NKCC1 Abcam antibody showing expression of wild-type NKCC1 and NKCC1-DFX in respective lanes. B: Western blot analysis of the cotransporter in brains from control NKCC1^WT/WT, mutant NKCC1^WT/DFX, and NKCC1^DFX/DFX mice. SPAK and actin antibodies were used to demonstrate equal loading. C: brain lysates from 3 mice of each genotype were collected and subjected to SDS-PAGE and probed with T4 and actin antibodies. D: quantitation of NKCC1 expression normalized to β-actin from samples from 6 mice. NKCC1 levels were significantly reduced in the NKCC1-DFX brain (n = 6, P < 0.05, t-test). NKCC1, Na⁺-K⁺-2Cl⁻ cotransporter-1; WT, wild type.