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. 2018 May 2;315(2):F395–F405. doi: 10.1152/ajprenal.00057.2018

Fig. 4.

Fig. 4.

Effects of folate-conjugated rapamycin (FC-rapa) versus unconjugated rapamycin on mammalian target of rapamycin (mTOR) signaling in kidney and spleen. A: immunoblot analysis of total kidney tissue lysates for phospho-ribosomal protein S6 (P-S6; Ser240/244), total S6, and β-actin as a loading control. B: immunoblot analysis of total spleen tissue lysates from treated mice for P-S6 (Ser240/244), total S6, and β-actin. For A and B, each lane represents a single animal that corresponds to all of the tissues shown, with 2 representative mice per treatment group (n = 5 for each treatment group) shown. Immunoblot results were quantified with the values representing the ratios of the P-S6 signals relative to the β-actin signals. C: immunoblot analysis of kidney tissue lysates from wild-type (WT) and treated animals for P-S6 (Ser240/244), total S6, phosphorylated 4e-binding protein 1 (P-4E-BP1; Thr37/46), total 4E-BP1, and β-actin.