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. 2018 Sep 15;4(9):e00788. doi: 10.1016/j.heliyon.2018.e00788

Fig. 3.

Fig. 3

Schematic of zebrafish mating history in this study. Original fish (F0) of each sex were fed BPA-containing food (0.1 mg of BPA/g food) or control food (BPA-non-containing food). After 4 to 6 months of treatment, F0 fish were mated with untreated fish (housed separately from experimental fish) and offspring (the F1 generation) were raised on control food. Fish were separated into four groups, with fish originating from F0 females treated with or without BPA and from F0 males treated with or without BPA as indicated. After 4 to 6 months of culture, F1 fish were mated with untreated fish, and juveniles (the F2 generation) were raised on control food. After 4 to 6 months of culture, F2 fish were mated with untreated fish, and juveniles (the F3 generation) were raised on control food. Serial cultivation was continued through the F2 and F3 generations.