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. 2018 Sep 15;4(9):e00788. doi: 10.1016/j.heliyon.2018.e00788

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 7 — Adverse effects of BPA treatment on sperm morphology in the F2 zebrafish. (A) Sperm was collected by ejaculation and was observed after staining with DAPI. Sperm from F2 generation males from the control (Control) and BPA-treated (BPA) groups under bright field (B. field) and DAPI filter views. The scale bar indicates 10 μm. (B) The number of DAPI-positive heads of sperm or spermatozoa-like cells in 1 μl of suspensions from F2 generation males from control (Control) or BPA-treated (BPA) fish were counted (n = 3). **indicates a statistically significant difference between the values for the BPA-treated group and the control group at the P < 0.01 level.