Figure 2.

Polycomb proteins and associated histone modifications at the active and repressed CCND2 gene. A, schematic of the human CCND2 locus. Positions of PCR amplicons analyzed in B are shown below the coordinate scale. Note that amplicon 2 corresponds to the summit of MEL18 peak, and amplicon 5 corresponds to the CCND2 TSS (see Tables S4 and S5, for sequence information). B, ChIP profiles of MEL18, RING2, SUZ12, and H3K27me3 in NT2-D1 (left column) and TIG-3 (right column) cells. The immunoprecipitation of the ALX4 gene repressed by PcG in NT2-D1 and TIG-3 cells was used as positive control (for position of PCR amplicons see Fig. S11). The immunoprecipitation of a gene desert region (chromosome 12: 127711096–127713493; Hg19) was used as a negative control. Histograms for both are shown to the right of the each graph and are to the same y axis scale. All histograms and graphs show the average and the scatter (whiskers) between two independent experiments.