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. 2018 Aug 2;293(37):14285–14294. doi: 10.1074/jbc.RA118.002264

Figure 1.

Figure 1.

SPR analysis of LacI binding in the presence of MSH sliding clamps. A 5′-biotin 98-bp duplex DNA (supporting Table S1) containing a +dT (TaMutS) or G/T (EcMutS and HsMSH2-HsMSH6) mismatch was anchored to the surface of the streptavidin-coated SPR (Biacore) chip and the remaining end blocked by dig-antidig as described previously (38). Each curve is color-coded and comes in pairs ± LacI injection. Association curves were processed as described in supporting Fig. S1 and supporting information to extract kon·LacI. a, TaMutS (0, 20, 50, 100, 200, 500, and 800 nm) ± LacI (0.5 nm). Subtracted LacI association curve in the presence of TaMutS (middle) and calculated kon·LacI plotted against TaMutS concentration (right). b, EcMutS (0, 10, 30, 60, 100, and 200 nm) ± LacI (1.5 nm). LacI association curve in the presence of EcMutS (middle) and calculated kon·LacI plotted against EcMutS concentration (right). c, HsMSH2-HsMSH6 (0, 10, 30, 60, 100, and 200 nm) ± LacI (1.5 nm). Left and right, LacI association curve in the presence of HsMSH2- HsMSH6 (left) and calculated kon·LacI plotted against HsMSH2-HsMSH6 concentration (right).