Figure 2.

Inhibition of P. aeruginosa ICL activity by small molecules. A, CoA, oxaloacetate and pyruvate noncompetitively inhibit ICL. Shown is a Lineweaver–Burk transformation of ICL kinetic data obtained in the presence of no addition (control) or in the presence of oxaloacetate, pyruvate, or CoA. B, Lineweaver–Burk plot showing that succinate inhibits ICL uncompetitively, whereas phosphoenolpyruvate displays mixed inhibition. C, itaconate and nitropropionate inhibit ICL uncompetitively, whereas maleic acid displays mixed inhibition. The plots were generated using GraphPad Prism version 6. All small molecules (except the substrate) were added at 1 mm final concentration.