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Pyruvate, oxaloacetate, and glyoxylate strongly activate the isocitrate dehydrogenase activity of IDH. A, effect of pyruvate, oxaloacetate, and glyoxylate on the isocitrate dependence of IDH kinetics. B, effect of pyruvate, oxaloacetate, and glyoxylate on the NADP+ dependence of IDH kinetics. Each regulator was added at a 1 mm final concentration. Error bars, ± 1 S.D. from n = 3 replicates.
