Figure 2.

Set4 promotes survival during oxidative stress. A, 10-fold serial dilutions of WT (yEG001) and set4Δ (yEG322 and yEG325) strains grown to mid-log phase in YPD or YPD with 4 mm H₂O₂ for 30 min. B, 10-fold serial dilutions of WT (yEG001) and set4Δ (yEG322 and yEG325) strains spotted on YPD, YPD with 100 μm CdCl₂, or YPD with with 150 μm CdCl₂. C, percentage survival calculated from cfu of WT (yEG001) and set4Δ (yEG325) strains following treatment with 4 mm H₂O₂ for 30 min. The scatter plot shows the mean and S.D. (error bars) from three biological replicates. An unpaired t test was used to determine statistical significance. D, percentage survival of WT and set4Δ cells (yEG638, yEG639, yEG640, and yEG641) carrying either the empty pRS316 vector or pRS316-SET4, with SET4 under the control of its endogenous promoter, calculated from cfu on SC-URA following treatment with 20 mm H₂O₂ for 30 min. One-way analysis of variance was performed, followed by Turkey's post hoc test to determine statistical significance. E, 10-fold serial dilutions of strains carrying either pMN3 (empty) or pMN3-SET4 (yEG372 and yEG375, respectively) that were pretreated with 1 μm β-estradiol for 4–5 h, followed by exposure to 20 mm H₂O₂ for 30 min. F, percentage survival calculated from cfu of cells grown as in E, except that 50 nm β-estradiol was used to induce SET4, and plated on SC-URA. An unpaired t test was used to determine statistical significance. For all panels, asterisks represent p values as follows: *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001. ns, not significant.