Figure 6.

Set4-dependent regulation of ERG gene expression. A, RT-qPCR of ERG genes from WT (yEG001) and set4Δ (yEG322) strains grown in YPD and treated with 0.4 mm H₂O₂ for 30 min. Expression levels were normalized to SCR1. -Fold change relative to the WT strain without H₂O₂ treatment is shown. Error bars, S.E. from three biological replicates. B, RT-qPCR as in A except that cells were grown in SC medium, and H₂O₂ treatment was not performed. Asterisks, p values from unpaired t tests (**, p ≤ 0.01; ***, p ≤ 0.001).