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GAPDH heme binding is conserved in yeast and essential for controlling cell heme tone and delivery to nuclear Hap1p. A, sensor-detected labile heme levels in WT and tdh3Δ (GAPDH deletion) yeast strains with or without transfection with yeast and human GAPDH variants. Values represent mean ± S.E. n = 3. B, corresponding Hap1p activities in yeast. Values represent mean ± S.E. n = 3, *, p ≤ 0.02; **, p < 0.005; ***, p < 0.0001, two-tailed t test.
