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. 2018 Jul 16;293(37):14210–14223. doi: 10.1074/jbc.RA118.002414

Table 1.

Gene-specific primers used for qPCR analysis and cloning

F is forward, and R is reverse.

Name Primer sequences
hIAPP F: 5′-AGCTACACCCATTGAAAGTCATC-3′
R: 5′-GATGAGAGAATGGCACCAAAGT-3′
rIAPP F: 5′-AGCTGTTCTCCTCATCCTCT-3′
R: 5′-CTGTGTTGCACTTCCGTTTG-3′
Insulin F: 5′-TGTCCTTCTGCCATGGCCCT-3′
R: 5′-TTCACAAAGGCTGCGGCTGG-3′
FoxA2 F: 5′-TCTCCATCAACAACCTCATGTC-3′
R: 5′-GTAGTGCATCACCTGTTCGTAG-3′
PDX1 F: 5′-CCTTGTGCTCGGGTTATGTT-3′
R: 5′-ATCATCCCACTGCCAGAAAG-3′
MAFA F: 5′-GCGGAGAACGGTGATTTCTA-3′
R: 5′-AGGAAAGGGAGGCTGAGAAG-3′
β-Actin (human) F: 5′-AGGCACCAGGGCGTGAT-3′
R: 5′-GCCCACATAGGAATCCTTCTGAC-3′
β-Actin (rat) F: 5′-GCTACAGCTTCACCACCACA-3′
R: 5′-ATCGTACTCCTGCTTGCTGA-3′
Human IAPP promoter-cloning primer F: 5′-AAAAAGATCTACAGCTCTGGCATTTATAAC-3′
R: 5′-AAAACCATGGCTTTTAATGTTTCAATGTCAGC-3′
FoxA2-binding region in rIAPP promoter-ChIP primer set F: 5′-GCACAAAATGGAAACTCCAAA-3′
R: 5′-CCCTCAACTTGCTTAGCTCTG-3′