Figure 2.
Synthetic peptides from N- and C-terminal regions. A, amino acid sequences of the synthesized N-terminal peptides that span the predicted HS-binding domains of BMP2 to BMP7. Peptides were N-terminally linked to a biotin molecule via a triple glycine linker. Kd values (in nanomolar) and pI values of synthetic peptides are shown, as well as the accession numbers for the full-length proteins from which the respective peptides were derived. B, amino acid sequences of the synthesized C-terminal peptides spanning putative HS-binding domains of BMP4, BMP5, and BMP6/7. Note that the C-terminal regions of BMP6 and BMP7 are completely identical. C, schematic of a representative BMP protein. The encoded and newly synthesized protein consists of a signal peptide (SP, green), a prodomain (yellow), and the mature biologically-active ligand (blue). A furin cleavage site separates the prodomain from the mature ligand, which contains seven conserved cysteines forming three intramolecular disulfide bonds and one intermolecular disulfide bond.