Table 1.

Comparative table of the Michaelis–Menten–derived kinetic parameters of choline oxidation and 18:1 LPC and 18:1 NBD-PEG4 LPE cleavage with respect to those reported for 12:0 lyso-NBD-PC (25)

Kinetic parameters	Choline oxidase	18:1 LPC	NBD-PEG4 LPE	NBD-LPC
Km (μm)	121 ± 23	112 ± 22	0.88 ± 0.09	308 ± 195
kcat (s−1)	1.32 ± 0.04	0.016 ± 0.01	0.021 ± 0.003	0.06 ± 0.01
kcat/Km (μm−1 s−1)	1.08 × 10−2 ± 1.0*10−4	2.1 × 10−4 ± 1.0 × 10−5	2.38 × 10−2 ± 2 × 10−4	1.9 × 10−4 ± 7 × 10−5