Table 2.

Kinetic parameters of the KinTek model

The confidence contours of each parameter depicted in Fig. 8 were calculated employing FitSpace statistical analysis on KinTek at a χ² > 0.9901 (Fig. 6).

Kinetic parameters	Parameter contour	Determined by	Derived parameters	Parameter contour
kLPC (μm−1 s−1)	100	Diffusion-limited	KD-LPC1 (μm)	[1.56, 1.92]
k−LPC1 (s−1)	[155.5, 191.7]	Model
k2 slow (s−1)	[0.011, 0.014]	Model	kcat slow (s−1)	[0.011, 0.014]
k−2 slow (μm−1 s−1)	0	Non-reversible
kLPC (μm−1 s−1)	100	Diffusion-limited	KD-LPC2 (μm)	[0]
k−LPC2 (s−1)	∼0	Model
kLPAo (μm−1 s−1)	100	Diffusion-limited	KD-LPAo (μm)	[0.069, 0.077]
k−LPAo (s−1)	[6.87, 7.73]	pNP-TMP competition
kLPAa (μm−1 s−1)	100	Diffusion-limited	KD-LPAa (μm)	[1.52, 2.77]
k−LPAa (s−1)	[151.7, 276.7]	Model
k2 fast (s−1)	[0.039, 0.048]	Model	kcat fast (s−1)	[0.039, 0.048]
k−2 fast (μm−1 s−1)	0	Non-reversible