Table 3.
Kinetic parameters defining the hydrolysis of different LPC species in the presence of their activating homologous LPA species fitted in the kinetic model (Fig. 8)
| LPC/LPA species | KD-LPC1 | KD-LPAo | KD-LPAa | kcat slow | kcat fast | kcat fast/kcat slow |
|---|---|---|---|---|---|---|
| μm | nm | μm | s−1 | s−1 | ||
| 14:0 | 0.54 ± 0.08 | 62 ± 8 | 3.6 ± 0.2 | 0.067 ± 0.021 | 0.10 ± 0.05 | 1.5 |
| 16:0 | 0.7 ± 0.1 | 50 ± 3 | 2.5 ± 0.1 | 0.018 ± 0.011 | 0.21 ± 0.13 | 11.8 |
| 18:0 | 2.36 ± 0.03 | 56 ± 9 | 1.31 ± 0.6 | 0.020 ± 0.009 | 0.18 ± 0.05 | 8.8 |
| 18:1 | 1.71 ± 0.36 | 72 ± 9 | 2.05 ± 1.25 | 0.013 ± 0.003 | 0.043 ± 0.009 | 3.3 |
| 22:0/22:4 | 3.08 ± 0.05 | 38 ± 6 | 1.08 ± 0.15 | 0.004 ± 0.018 | 0.008 ± 0.003 | 2.1 |