Figure 5.

Altered mobility of α-DG in COG-deficient CHO cells is a function of COG-dependent defects in O-glycosylation. A, representative Western blotting of WT CHO and ldlB cell lysates with or without PNGase F digestion (n = 2). The cells were treated with or without V. cholerae neuraminidase (120 milliunits/ml) prior to lysis. B, cells were cultured with increasing concentrations of a furin inhibitor, and Western blotting analysis was performed on lysates using an anti-DG (core) antibody (n = 2). Furin inhibition results in a comparable increase in the apparent molecular weight of α-DG in both WT CHO and ldlB cells. C, cells were treated with two different amounts (60 or 120 milliunits/ml) of V. cholerae neuraminidase, and Western blotting analysis was performed. D, Western blotting of α-DG in WT, ldlB, and cog1-corrected ldlB cells treated with or without V. cholerae neuraminidase (n = 2). IB, immunoblot.