Figure 4.
cAMP suppresses AChE expression through CRE site. A, cultured B16F10 cells transfected with pAChE-Luc or pCRE-Luc constructs were treated with various concentrations of Bt2-cAMP for 12 h, and then cell lysates were collected for luciferase assay. B, the mutagenesis of CRE site on pAChE-Luc is shown. C, cultured B16F10 cells transfected with pAChE-Luc or pAChEΔCRE-Luc construction were treated with various concentrations of Bt2-cAMP for 12 h, and then cell lysates were collected for luciferase assay. Data are normalized and expressed as the percentage of control. All values are in mean ± S.D., n = 4, each with triplicate samples; *, p < 0.05; **, p < 0.01.