Figure 2.

Binding of cMyBP-C N-terminal domains to NTF and myosin S2Δ. A, NTF co-sedimentation binding data for C1mC2 (red), C1mC2–3P (orange), and C1mC2–3SD (purple). B, normalized MST binding curves for C1mC2 (red), isolated m-motif (green), or C1–C2 (blue) titrated against myosin S2Δ. C, normalized MST curves for C1mC2 (red) and m-motif (green) binding to myosin S2Δ in the presence of 50 mm KCl (circles) and 180 mm KCl (squares). D, binding of C1mC2 (red), C1mC2–3P (orange), and C1mC2–3SD (purple) to myosin S2Δ. For all experiments, means ± S.E., n = 4–8.