Table 2.
Primer sequences and annealing temperatures used for mRNA and miRNA quantification by real-time RT-PCR
| Target | Forward Primer Sequence (5′–3′) | Reverse Primer Sequences (3′–5′) | Annealing Temperature, °C | Reference No. |
|---|---|---|---|---|
| srebp1c | GACAAGGTGGTCCCAGTTGCT | CACACGTTAGTCCGCATCAC | 58 | (68a) |
| fasn | TGATCTGAAGGCCCGTGTCA | GGGTGACGTTGCGTGGTAT | 58 | (58) |
| acly | GCTTTTGCCACGGTGGTCTC | GCTTCCGCTACGCCAATGTC | 60 | (76a) |
| cpt1a | TCGATTTTCAAGGGTCTTCG | CACAACGATCAGCAAAGTGG | 57 | (76a) |
| srebp2 | TAGGCCCCAAAGGGATAAG | TCAGACACGACGAGCACAA | 58 | (58) |
| hmgcs | AGTGGCAAAGAGAGGGTGTG | TTCTGGTTGGAGACGAGGAG | 60 | (58) |
| lxr | TGCAGCAGCCGTATGTGGA | GCGGCGGGAGCTTCTTGTC | 60 | (14a) |
| ugt1a3 | CCACCAGCAAGACAGTCTCA | CAACAGCACAGTGGCTGACT | 61 | (58) |
| ef1a | CATTGACAAGAGAACCATTGA | CCTTCAGCTTGTCCAGCAC | 56 | (1a) |
| 18S | GGCGGCGTTATTCCCATGA | TGCCCTTCCGTCAATTCCTTTA | 60 | (45) |
| drosha | GAGGAGTCGGTGAAGGAATG | CATGTGGGAGAAGAGGGAGA | 60 | Newly designed |
| miR-122 | TGGAGTGTGACAATGGTGTTTG | Universal reverse primer | 60 | (58) |
| miR-33 | GTGCATTGTAGTTGCATTGCAT | Universal reverse primer | 58 | (58) |
| Sno-U23 | GCCCATGTCTGCTGTGAAACAAT | Universal reverse primer | 60 | (76) |
srebp1c, Sterol regulatory element-binding protein 1c; fasn, fatty acid synthase; acly, ATP citrate lyase; cpt1a, carnitine palmitoyltransferase 1A; hmgcs, hydroxymethylglutaryl-CoA synthase; lxr, liver X receptor; ugt1a3, UDP glucuronosyltransferase family 1 member A3; srebp2, sterol regulatory element-binding protein 2.