Fig. 3.

Differential regulation of Angptl4 gene expression and protein secretion. Gene expression for Angptl4 in rat tibialis anterior (A) and differentiated L6 myotube cells (B) are reported as mean fold change (± SE), and ANGPTL4 protein levels in serum (C) and L6 myotube secretion media (D) are reported as ng/µl. The LC (A, C) and the BSA control (B, D) are represented with a dotted line, and were arbitrarily set to 1 for gene expression (A, B). Statistical significance was determined by one-way ANOVA (A, C, and D) or two-way ANOVA (B), followed by a post hoc least significant difference test. Bars sharing letters are not statistically significant from one another. *Statistical difference (P < 0.05) compared with LC (A, C) or BSA control (B, D). Rat work, n = 8 per group. L6 work, n = 3–6 per condition.