Table 2.
Proteins encoded by differentially expressed genes predicted by at least one method to contain a signal peptide sequence
| Signal Peptide Prediction Method |
|||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Entrez Gene ID | RefSeq Protein ID | Signal-BLAST | TOPCONS2 | SignalP 4.1 | UniProt Evidence of Secretion |
| Acacb | Acetyl-CoA carboxylase beta | 116719 | NP_446374.1 | ● | ● | ||
| Acadl | Acyl-CoA dehydrogenase, long chain | 25287 | NP_036951.1 | ● | |||
| Adam9 | ADAM metallopeptidase domain 9 | 290834 | NP_001014772.1 | ● | ● | ● | ● |
| Angptl2 | Angiopoietin-like 2 | 171100 | NP_598253.1 | ● | ● | ● | ● |
| Angptl4 | Angiopoietin-like 4 | 362850 | NP_954546.1 | ● | ● | ● | ● |
| Bcam | Basal cell adhesion molecule | 78958 | NP_113940.1 | ● | ● | ● | |
| Casq | Calsequestrin 2 | 29209 | NP_058827.3 | ● | ● | ● | |
| Cd34 | CD34 molecule | 305081 | NP_001100672.1 | ● | ● | ● | |
| Col15a1 | Collagen type XV alpha 1 chain | 298069 | NP_001094005.1 | ● | ● | ● | ● |
| Col3a1 | Collagen type III alpha 1 chain | 84032 | NP_114474.1 | ● | ● | ● | ● |
| Fbxl4 | F-box and leucine-rich repeat protein 4 | 313101 | NP_001101389.1 | ● | |||
| Gpihbp1 | Glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1 | 300027 | NP_001124019.1 | ● | ● | ● | |
| Gscsh | Glycine cleavage system protein H | 171133 | NP_598282.2 | ● | |||
| Hmcn1 | Hemicentin 1 | 289094 | NP_001258221.1 | ● | ● | ● | ● |
| Igf1 (isoform a) | Insulin-like growth factor 1 | 24482 | NP_001075946.2 | ● | ● | ● | ● |
| Igf1 (isoform b) | Insulin-like growth factor 1 | 24482 | NP_849197.3 | ● | ● | ● | ● |
| Igf1 (isoform c) | Insulin-like growth factor 1 | 24482 | NP_001075947.1 | ● | ● | ● | ● |
| Igf1 (isoform d) | Insulin-like growth factor 1 | 24482 | NP_001075948.1 | ● | ● | ● | ● |
| Lgi1 | Leucine-rich, glioma inactivated 1 | 252892 | NP_665712.1 | ● | ● | ● | ● |
| Lyz2 | Lysozyme 2 | 25211 | NP_036903.2 | ● | ● | ● | ● |
| Nrp1 | Neuropilin 1 | 246331 | NP_659566.1 | ● | ● | ● | ● |
| Ntn4 | Netrin 4 | 299737 | NP_001100250.1 | ● | ● | ● | ● |
| Pdgfd | Platelet derived growth factor D | 66018 | NP_076452.1 | ● | ● | ● | ● |
| Serpinh1 | Serpin family H member 1 | 29345 | NP_058869.1 | ● | ● | ● | |
| St3gal2 | ST3 beta-galactoside alpha-2,3-sialyltransferase 2 | 64442 | NP_113883.2 | ● | ● | ● | |
| Tm2d2 | TM2 domain containing 2 | 290833 | NP_001017444.1 | ● | ● | ||
| Vwa7 | Von Willebrand factor A domain containing 7 | 309611 | NP_997664.1 | ● | ● | ● | ● |
| Wnt16 | Wnt family member 16 | 500047 | NP_001102693.1 | ● | ● | ● | ● |
| Total | 23 | 24 | 28 | 18 | |||
SignalP 4.1 was used with sensitive settings as opposed to the default settings, which reduced the number of false negatives. Default settings were used for Signal-BLAST and TOPCONS2. Genes in boldface (n = 18, 15 unique) were those with evidence of secretion as per UniProt, used for microarray validation, and investigated for potentially being affected by polyunsaturated fatty acid treatment.