Table 3.
Differentially expressed genes identified by microarray and validated by qRT-PCR
| Entrez Gene ID | Gene Symbol | Microarray | qRT-PCR |
|---|---|---|---|
| 290834 | Adam9 | −1.22 | −1.58* |
| 171100 | Angptl2 | −1.40 | −1.27 |
| 362850 | Angptl4 | 6.54 | 17.13* |
| 298069 | Col15a1 | −1.86 | −5.05* |
| 84032 | Col3a1 | −1.75 | −2.67* |
| 289094 | Hmcn1 | −1.19 | −2.80* |
| 24482 | Igf1 | −1.71 | −2.04* |
| 252892 | Lgi1 | −2.77 | −7.24* |
| 25211 | Lyz2 | 2.00 | 1.77* |
| 246331 | Nrp1 | −1.48 | −1.53* |
| 299737 | Ntn4 | −1.41 | −4.41* |
| 66018 | Pdgfd | −1.37 | −2.17* |
| 64442 | St3gal2 | 1.23 | 1.64* |
| 309611 | Vwa7 | −1.33 | −1.78* |
| 500047 | Wnt16 | −1.71 | −1.69* |
Results correspond to fold changes for genes encoding secreted proteins that differed significantly between obese control (OC) vs. lean control (LC) rats (n = 8 per diet group).
Statistically significant quantitative (q)RT-PCR fold change (P < 0.05).