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Localization of Nkx3-2 expression in the kidney by RNAScope probes. Representative images from 6, 12, and 18 mo old animals show Nkx3-2 expression (magenta) in most cells of the kidney including all cells in the glomerulus. In addition to probes for Nkx3-2, we used probes to identify the proximal tubule (Ggt, green) and the collecting duct (Aqp2, yellow). The white line indicates the outline of the glomerulus.
