Fig. 2.

Systemic levels of IL 1-α, IL-1-β, IL-6, IL-10, TNF-α, IL-17, and IFN-γ in mice after ITE treatment. After euthanasia, serum levels of IL 1-α, IL-1-β, IL-6, IL-10, TNF-α, IL-17, and IFN-γ were determined by Bio-Rad ELISA multiplex kit, which is capable of detecting >15 pg/ml of these analytes. The data presented are the mean concentrations of IL 1-α, IL-1-β, IL-6, -IL-10, TNF-α, IL-17, and IFN-γ ± SE from 3 separate repeated experiments that include 6 mice each (n = 18). The statistical significance between cytokine levels for various groups was assessed by ANOVA. Statistically significant differences (*P < 0.01) between DSS + vehicle and DSS + ITE groups. DSS, dextran sodium sulfate; ITE, 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester.