Fig. 4.

ITE treatment differentially mediates macrophages and dendritic cells (DCs). Spleen, MLNs, and cLP lymphocytes were isolated from all 4 groups of 6 mice each in 3-repeated experiments (n = 18) at the experimental end point. In this experiment, we added naive-treated with ITE alone without any exposure to determine the independent effect of ITE without colitis. Changes in the frequency and expression of macrophages (F4/80) and DCs (CD11c) from spleens, MLNs, and cLP are shown (A). Changes in the number of macrophages (F4/80) and DCs (CD11c) are shown (B and C, respectively). The statistical significance in flow cytometry data among 4 groups was assessed by ANOVA. *Statistically significant differences between DSS + vehicle and DSS + ITE groups; i.e.; *P < 0.01. Representative data of one of at least 3 experiments that produced similar results are shown. cLP, colon lamina propria; DSS, dextran sodium sulfate; ITE, 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester; MLN, mesenteric lymph node.