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Intestinal cytosolic phosphoenolpyruvate carboxykinase (Pck1) is responsive to feeding and fasting and is lost in small intestine PEPCK knockout (SIPKO) mice. A: quantitative real-time PCR of Pck1 mRNA in the duodenum (D), jejunum (J), and ileum (I) from fed and 24-h fasted control and SIPKO mice. B: Western blot analysis of PEPCK protein content in response to feeding and fasting. *P < 0.05 by t-test, n = 3 mice. PEPCK; phosphoenolpyruvate carboxykinase.
