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. 2018 Apr 6;315(2):G249–G258. doi: 10.1152/ajpgi.00039.2018

Fig. 5.

Fig. 5.

Cystolic phosphoenolpyruvate carboxykinase (Pck1) deletion causes a buildup of jejunum TCA cycle metabolites and an oxidized redox state. Jejunum organic acids in fed (A) and 24-h fasted (B) mice; n = 8. Mitochondrial redox state represented by the αKG x NH4/Glu ratio; n = 7 (C). Cytosolic redox state represented by the lactate/pyruvate ratio; n = 8 (D). Oxygen consumption by isolated villus cells incubated in modified DMEM/F-12 containing 5 mM glutamine and 5 mM glucose; n = 5 (E). Total intestine length; n = 15 (F). Hematoxylin-eosin (H&E) staining of jejunum slices (G). *P < 0.05 by two-tailed t-test. n = no. of mice. αKG, α-ketoglutarate; Cit, citrate; Fum, fumarate; Lac, lactate; OAA, oxaloacetate; Pyr, pyruvate; Succ, succinate.