Fig. 3.

Dose-response effect of cholecystokinin (CCK)-8 and CCK-JMV to stimulate p21-activated kinase (PAK)4 phosphorylation in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of CCK-8 and CCK-JMV (at the indicate concentrations) for 3 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 antibody and, as loading control, anti-total PAK4. Top: results of a representative blot of three independent experiments. Bottom: means ± SE of three independent experiments. Results are expressed as % of basal stimulation of the control group (CCK-8 1 nM: 193 ± 15% of control). *P < 0.05 compared with the control group.