Fig. 9.

Ability of the p21-activated kinase (PAK) inhibitor II, LCH-7749944 (LCH), to inhibit cholecystokinin (CCK)-8 or 12-O-tetradecanoylphobol-13-acetate (TPA) activation of PAK4 (A) or PAK2 (B). Isolated pancreatic acini were preincubated for 1 and 3 h in the presence or absence of LCH-7749944 (at the indicated concentrations) and then treated with CCK-8 (1 nM) for 3 min and TPA (1 µM) for 5 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pT402 PAK2 antibody and, as loading control, anti-total PAK4 or anti-total PAK2. Top of A and B: results of a representative blot of three independent experiments. Bottom of A and B: means ± SE for at least five independent experiments. Results are expressed as % of basal stimulation of the control group. *P < 0.05 compared with the control group, ∞P < 0.05 compared with CCK-8 alone, and ∆P < 0.05 compared with TPA alone.